Israel Hanukoglu, Ph. D.
Department of Molecular Biology
Ariel University Center in Samaria
Ariel 44837, Israel

NOTE: Full text REPRINTS of some recent publications are available below. Scroll down to see the hyperlinks.

• Publications ordered by citation frequency
• Mitochondrial P450 systems involved in steroid hormone biosynthesis (steroidogenesis)
• Pseudohypoaldosteronism and Epithelial Sodium Channels (ENaC)
• Cytoskeletal Proteins - Keratins, and Actin

1. Prostaglandins as first mediators of stress [letter].

Hanukoglu I. N Engl J Med 296:1414-1414, 1977.

2. Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat.

Hanukoglu I., Karavolas H.J., and Goy R.W. Brain Res 125:313-324, 1977.
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3. Molecular models of anesthetic drug action - variations on a theme [letter].

Hanukoglu I. Anesthesiology 47:532-534, 1977.

4. Pregnenolone separation from cholesterol using Sephadex LH-20 minicolumns

Hanukoglu I., and Jefcoate C.R. J Chromatogr 190:256-262, 1980.
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5. Mitochondrial cytochrome P-450scc. Mechanism of electron transport by adrenodoxin.

Hanukoglu I., and Jefcoate C.R. J Biol Chem 255:3057-3061, 1980.
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6. Mechanism of electron transport to cytochrome P-450 in adrenal cortex mitochondrial monooxygenase systems

Hanukoglu I. Ph. D. Dissertation, University of Wisconsin- Madison. , 1980.

7. Adrenal mitochondrial cytochrome P-450scc. Cholesterol and adrenodoxin interactions at equilibrium and during turnover.

Hanukoglu I., Spitsberg V., Bumpus J.A., Dus K.M., and Jefcoate C.R. J Biol Chem 256:4321-4328, 1981.
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8. Mechanisms of ionic activation of adrenal mitochondrial cytochromes P-450scc and P-45011 beta.

Hanukoglu I., Privalle C. T., and Jefcoate C. R. J Biol Chem 256:4329-4335, 1981.
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9. The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.

Hanukoglu I., and Fuchs E. Cell 31:243-252, 1982.
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10. Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.

Hanukoglu I., Tanese N., and Fuchs E. J Mol Biol 163:673-678, 1983.
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11. The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins.

Hanukoglu I., and Fuchs E. Cell 33:915-924, 1983.
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12. Unraveling the structure of the intermediate filaments.

Fuchs E., and Hanukoglu I. Cell 34:332-334, 1983.

13. The evolution and complexity of the genes encoding the cytoskeletal proteins of human epidermal cells.

Fuchs E., Kim K.H., Hanukoglu I., and Tanese N. Curr Probl Dermatol 11:27-44, 1983.

15. The influence of differential keratin gene expression on the structure and properties of the resulting 8 nm filaments

Fuchs E., Kim K.H., Hanukoglu I., Marchuk D., Tyner A., and McCrohon S. Molecular Biology of the Cytoskeleton. Cold Spring Harbor, pp. 381-393, 1984.

16. The nature and significance of differential keratin gene expression.

Fuchs E., Hanukoglu I., Marchuk D., Grace M. P., and Kim K.H. Ann N Y Acad Sci 455:436-450, 1985.

17. Epidermal a-keratins: structural diversity and changes during tissue differentiation

Fuchs E., and Hanukoglu I. Biology of the Integument vol 2, (Bereiter-Hahn, J., Matoltsy, A. G., and Richards, K. S. eds.) Springer-Verlag, Berlin, pp. 644-665, 1986.

18. Structural diversity and evolution of intermediate filament proteins

Hanukoglu I., and Fuchs E. Evolutionary Processes and Theory, (Karlin, M., and Nevo, E. eds.), Academic Press, pp. 69-98, 1986.

19. Stoichiometry of mitochondrial cytochromes P-450, adrenodoxin and adrenodoxin reductase in adrenal cortex and corpus luteum: Implications for membrane organization and gene regulation.

Hanukoglu I., and Hanukoglu Z. Eur J Biochem 157:27-31, 1986.
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20. Isolation of a cDNA for adrenodoxin reductase (ferredoxin-NADP+ reductase). Implications for mitochondrial cytochrome P-450 systems.

Hanukoglu I., Gutfinger T., Haniu M., and Shively J.E. Eur J Biochem 169:449-455, 1987.
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21. Human adrenodoxin reductase: two mRNAs encoded by a single gene on chromosome 17cen-q25 are expressed in steroidogenic tissues.

Solish S. B., Picado-Leonard J., Morel Y., Kuhn R. W., Mohandas T.K., Hanukoglu I., and Miller W.L. Proc Natl Acad Sci U S A 85:7104-7108, 1988.
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22. cDNA sequence of adrenodoxin reductase. Identification of NADP-binding sites in oxidoreductases.

Hanukoglu I., and Gutfinger T. Eur J Biochem 180:479-484, 1989.
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23. Olfactory-specific cytochrome P-450. cDNA cloning of a novel neuroepithelial enzyme possibly involved in chemoreception.

Nef P., Heldman J., Lazard D., Margalit T., Jaye M., Hanukoglu I., and Lancet D. J Biol Chem 264:6780-6785, 1989.
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24. Molecular biology of cytochrome P450 systems in steroidogenic tissues

Hanukoglu I. Serono Symposia Review 23:233-252, 1989.

25. Elimination of non-specific binding in western blots from non-reducing gels.

Hanukoglu I. J Biochem Biophys Methods 21:65-68, 1990.
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26. Induction and mitochondrial localization of cytochrome P450scc system enzymes in normal and transformed ovarian granulosa cells.

Hanukoglu I., Suh B.S., Himmelhoch S., and Amsterdam A. J Cell Biol 111:1373-1381, 1990.
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27. Mechanism of corticotropin and cAMP induction of mitochondrial cytochrome P450 system enzymes in adrenal cortex cells.

Hanukoglu I., Feuchtwanger R., and Hanukoglu A. J Biol Chem 265:20602-20608, 1990.
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28. Adrenodoxin reductase of mitochondrial cytochrome P450 systems: structure and regulation of expression

Hanukoglu I. Flavins and Flavoproteins 1990 (Curti, B., Ronchi, S., and Zanetti, G., Eds.). pp. 859-864, 1991.

29. Rapid preparation of lambda phage DNA

Raikhinstein M., and Hanukoglu I. Meth. Mol. Cell. Biol. 3:108-111, 1992.

30. Reductases of the bacterial, mitochondrial and microsomal cytochrome P450 systems: structural comparisons

Hanukoglu I. Cytochrome P-450: Biochemistry and Biophysics (A.I. Archakov and G.I. Bachmanova, eds.), INCO-TNC, Moscow, pp. 339-344, 1992.

31. Current research on steroid metabolism: transition from biochemistry to molecular-cell biology.

Hanukoglu I. J Steroid Biochem Molec Biol 43:745-749, 1992.
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32. Steroidogenic enzymes: structure, function, and role in regulation of steroid hormone biosynthesis.

Hanukoglu I. J Steroid Biochem Molec Biol 43:779-804, 1992.
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33. Oncogene-transformed granulosa cells as a model system for the study of steroidogenic processes.

Amsterdam A., Hanukoglu I., Suh B.S., Keren-Tal I., Plehn-Dujowich D., Sprengel R., Rennert H., and Strauss III J.F. J Steroid Biochem Molec Biol 43:875-884, 1992.
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34. A molecular view of steroid metabolism

Hanukoglu I. (editor) J Steroid Biochem Molec Biol 43:745-1124, 1992.

35. Proceedings of the 9th International Symposium on Microsomes and Drug Oxidations

Kapitulnik J., and Hanukoglu I. (editors). J Basic Clin Physiol Pharmacol 3:1-309, 1992.

36. Electron leakage from the mitochondrial NADPH-adrenodoxin reductase-adrenodoxin-P450scc (cholesterol side chain cleavage) system.

Hanukoglu I., Rapoport R., Weiner L., and Sklan D. Arch Biochem Biophys 305:489-498, 1993.
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37. Mitochondrial-genome-encoded RNAs: differential regulation by corticotropin in bovine adrenocortical cells.

Raikhinstein M., and Hanukoglu I. Proc Natl Acad Sci U S A 90:10509-10513, 1993.
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38. Cloning of LL5, a novel protein encoding cDNA from a rat pituitary library.

Levi L., Hanukoglu I., Raikhinstein M., Kohen F., and Koch Y. Biochim Biophys Acta 1216:342-344, 1993.
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39. cDNA cloning and sequence analysis of the bovine adrenocorticotropic hormone (ACTH) receptor.

Raikhinstein M., Zohar M., and Hanukoglu I. Biochim Biophys Acta 1220:329-332, 1994.
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40. A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems.

Rapoport R., Hanukoglu I., and Sklan D. Anal Biochem 218:309-313, 1994.
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41. Cloning of ACTH-regulated genes in the adrenal cortex.

Raikhinstein M., and Hanukoglu I. J Steroid Biochem Mol Biol 49:257-260, 1994.
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42. Electron pathways in adrenal mitochondrial cytochrome P450 systems: Relative rates of leakage and hydroxylation

Rapoport R., Raikhinstein M., Sklan D., and Hanukoglu I. Cytochrome P-450: Biochemistry Biophysics and Molecular Biology (M. C. Lechner, ed.). John Libbey Eurotext, Paris, pp. 357-363, 1994.

43. Electron leakage from the adrenal cortex mitochondrial P450scc and P450c11 systems: NADPH and steroid dependence.

Rapoport R., Sklan D., and Hanukoglu I. Arch Biochem Biophys 317:412-416, 1995.
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44. Routes and regulation of NADPH production in steroidogenic mitochondria.

Hanukoglu I., and Rapoport R. Endocr Res 21:231-241, 1995.
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45. Selective increases in adrenal steroidogenic capacity during acute respiratory disease in infants.

Hanukoglu A., Fried D., Nakash I., and Hanukoglu I. Eur J Endocrinol 133:552-556, 1995.
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46. Exclusion of the locus for autosomal recessive pseudohypoaldosteronism type 1 from the mineralocorticoid receptor gene region on human chromosome 4q by linkage analysis.

Chung E., Hanukoglu A., Rees M., Thompson R., Dillon M., Hanukoglu I., Bistritzer T., Kuhnle U., Seckl J., and Gardiner R.M. J Clin Endocrinol Metab 80:3341-3345, 1995.
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47. Tissue- and species-dependent expression of sheep lung microsomal cytochrome P4502B(LgM2).

Arinc E., Hanukoglu I., Sen A., and Adali O. Biochem Mol Biol Int 37:1121-1126, 1995.

48. Biyokimyadan molekuler hucre biyolojisine (In Turkish).

Hanukoglu I. Populer Bilim (Popular Science) 2:45-46, 1995.

49. Bir bilimadaminin 20 yillik ozlemi. (In Turkish). (English: Twenty years longing of a scientist - an autobiographical essay after a visit to Turkey to attend a scientific meeting)

Hanukoglu I. Populer Bilim (Popular Science) 2:47-49, 1995.

50. Localisation of pseudohypoaldosteronism genes to chromosome 16p12.2-13.11 and 12p13.1-pter by homozygosity mapping.

Strautnieks S. S., Thompson R. J., Hanukoglu A., Dillon M.J., Hanukoglu I., Kuhnle U., Seckl J., Gardiner R. M., and Chung E. Hum Mol Genet 5:293-299, 1996.
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51. Mutations in subunits of the epithelial sodium channel cause salt wasting with hyperkalaemic acidosis, pseudohypoaldosteronism type 1.

Chang S.S., Grunder S., Hanukoglu A., Rosler A., Mathew P.M., Hanukoglu I., Schild L., Lu Y., Shimkets R.A., Nelson-Williams C., Rossier B.C., and Lifton R.P. Nat Genet 12:248-253, 1996.
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52. Electron transfer proteins of cytochrome P450 systems

Hanukoglu I. Adv Mol Cell Biol 14:29-55, 1996.
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53. Pseudohypoaldosteronism due to renal and multisystem resistance to mineralocorticoids respond differently to carbenoxolone.

Hanukoglu A., Joy O., Steinitz M., Rosler A., and Hanukoglu I. J Steroid Biochem Mol Biol 60:105-112, 1997.
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54. Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle.

Rapoport R., Sklan D., Wolfenson D., Shaham-Albalancy A., and Hanukoglu I. Biochim Biophys Acta 1380:133-140, 1998.
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55. In situ localization of ACTH receptor-like mRNA in molluscan and human immunocytes.

Ottaviani E., Franchini A., and Hanukoglu I. Cell Mol Life Sci 54:139-142, 1998.
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56. Gene structure of the human amiloride-sensitive epithelial sodium channel beta subunit.

Saxena A., Hanukoglu I., Strautnieks S.S., Thompson R.J., Gardiner R.M., and Hanukoglu A. Biochem Biophys Res Commun 252:208-213, 1998.
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57. Structures of mitochondrial P450 system proteins

Hanukoglu I. Molecular and applied aspects of oxidative drug metabolizing enzymes (E. Arinc, E. Hodgeson and J.B. Schenkman, eds.) Plenum Publishing Corp. pp. 41-54, 1999.

58. Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.

Vonrhein C., Schmidt U., Ziegler G.A., Schweiger S., Hanukoglu I., and Schulz G.E. FEBS Lett 443:167-169, 1999.
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59. The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.

Ziegler G.A., Vonrhein C., Hanukoglu I., and Schulz G.E. J Mol Biol 289:981-990, 1999.
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60. Structure and function of bovine adrenodoxin reductase

Ziegler G.A., Vonrhein C., Hanukoglu I., and Schulz G.E. Flavins and Flavoproteins (Ghisla S., Kroneck P., Macheroux P., Sund H., eds.), Rudolf Weber, Berlin, pp. 729-736, 1999.

61. Structures of adrenodoxin reductase and adrenodoxin support shuttle mechanism of electron transfer in mitochondrial P450 systems

Ziegler G.A., Vonrhein C., Schulz G.E., and Hanukoglu I. Molecular Steroidogenesis (M. Okamoto, Y. Ishimura, H. Nawata, eds.), Universal Academy Press, Tokyo, Japan, pp. 61-64, 2000.
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62. Aldosterone unresponsiveness (pseudohypoaldosteronism) results from mutations in epithelial sodium channel subunits

Hanukoglu A., Saxena A., Gardiner R.M., and Hanukoglu I. Molecular Steroidogenesis (M. Okamoto, Y. Ishimura, H. Nawata, eds.), Universal Academy Press, Tokyo, Japan, pp. 369-370, 2000.

63. Aldosterone insensitivity syndromes (pseudohypoaldosteronism): from clinic to molecular biology of sodium transport

Hanukoglu A., Saxena A., and Hanukoglu I. Pol J Endocrinol 52 (Suppl. 1):27-37, 2001.

64. Amiloride-sensitive epithelial sodium channel (ENaC) subunits are expressed in human and mussel immunocytes.

Ottaviani E., Franchini A., Mandrioli M., Saxena A., Hanukoglu A., Hanukoglu I. Dev Comp Immunol 26:395-402, 2002.
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65. Novel mutations responsible for autosomal recessive multi-system pseudohypoaldosteronism and sequence variants in Epithelial Sodium Channel (ENaC) alpha, beta and gamma subunit genes.

Saxena A., Hanukoglu I., Saxena D., Thompson R. J., Gardiner R.M., and Hanukoglu A. J Clin Endocrinol Metab 87:3344-3350, 2002.
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66. ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells.

Reichenstein M., Reich R., LeHoux J.G., and Hanukoglu I. Mol. Cell. Endocrinol. 215:109-114, 2004.
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67. Novel mutations in epithelial sodium channel (ENaC) subunit genes and phenotypic expression of multi-system pseudohypoaldosteronism (Review).

Edelheit O., Hanukoglu I., Gizewska M., Kandemir N., Tenenbaum-Rakover Y., Yurdakok M., Zajaczek S., Hanukoglu A. Clin Endocrinol. 62:547-553, 2005.
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68. Antioxidant protective mechanisms against reactive oxygen species (ROS) generated by mitochondrial P450 systems in steroidogenic cells (Review).

Hanukoglu I. Drug Metab Rev 38:171-196, 2006.
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69. Renin-aldosterone response, urinary Na/K ratio and growth in pseudohypoaldosteronism patients with mutations in epithelial sodium channel (ENaC) subunit genes.

Hanukoglu A., Edelheit O., Shriki Y., Gizewska M., Dascal N., Hanukoglu I. J Steroid Biochem Mol Biol 111:268-274, 2008.
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70. Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies.

Edelheit O., Hanukoglu A., and Hanukoglu I. BMC Biotechnology 9:61, 2009.
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71. Truncated beta epithelial sodium channel (ENaC) subunits responsible for multi-system pseudohypoaldosteronism support partial activity of ENaC.

Edelheit O., Hanukoglu I., Shriki Y., Tfilin M., Dascal N., Gillis, D., Hanukoglu A. J Steroid Biochem Mol Biol 119:84-88, 2010.
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72. Clinical improvement in patients with autosomal recessive pseudohypoaldosteronism and the necessity for salt supplementation.

Hanukoglu A., Hanukoglu I. Clin Exp Nephrol 14:518-519, 2010.
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73. Autosomal recessive hyponatremia due to isolated salt wasting in sweat associated with a mutation in the active site of Carbonic Anhydrase 12.

Muhammad E., Leventhal N., Parvari G., Hanukoglu A., Hanukoglu I., Chalifa-Caspi V., Feinstein Y., Weinbrand J., Jacoby H., Manor E., Nagar T., Beck J.C., Sheffield V.C., Hershkovitz E., Parvari R. Hum Genet 129:397-405, 2011.

74. Identification of the roles of conserved charged residues in the extracellular domain of epithelial sodium channel (ENaC) subunit by alanine mutagenesis.

Edelheit O., Hanukoglu I., Dascal N., Hanukoglu A. Am J Physiol Renal Physiol 300:F887-F897, 2011.
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75. Epithelial sodium channels (ENaC) are uniformly distributed on motile cilia in the oviduct and the respiratory airways.

Enuka Y., Hanukoglu I., Edelheit O., Vaknine H., Hanukoglu A. Histochem Cell Biol 137:339-353, 2012.

Note: References 5 and 7 are the major references for cholesterol side-chain-cleaving cytochrome P450scc in the IUBMB Enzyme Nomenclature.