Israel Hanukoglu, Ph.D.
Laboratory of Cell Biology
Ariel University
Ariel 40700, Israel

Publications on P450 systems and steroidogenesis

(This is a PARTIAL list of publications of Prof. I. Hanukoglu. For other topics select one of the options on the left side menu.)

2. Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat.
Hanukoglu I., Karavolas H.J., and Goy R.W.
Brain Res 125:313-324, 1977. REPRINT

4. Pregnenolone separation from cholesterol using Sephadex LH-20 minicolumns.
Hanukoglu I., and Jefcoate C.R.
J Chromatogr 190:256-262, 1980. REPRINT

5. Mitochondrial cytochrome P-450scc. Mechanism of electron transport by adrenodoxin.
Hanukoglu I., and Jefcoate C.R.
J Biol Chem 255:3057-3061, 1980. REPRINT

6. Mechanism of electron transport to cytochrome P-450 in adrenal cortex mitochondrial monooxygenase systems.
Hanukoglu I.
Ph. D. Dissertation, University of Wisconsin-Madison :, 1980.

7. Adrenal mitochondrial cytochrome P-450scc. Cholesterol and adrenodoxin interactions at equilibrium and during turnover.
Hanukoglu I., Spitsberg V., Bumpus J.A., Dus K.M., and Jefcoate C.R.
J Biol Chem 256:4321-4328, 1981. REPRINT

8. Mechanisms of ionic activation of adrenal mitochondrial cytochromes P-450scc and P-45011 beta.
Hanukoglu I., Privalle C. T., and Jefcoate C. R.
J Biol Chem 256:4329-4335, 1981. REPRINT

19. Stoichiometry of mitochondrial cytochromes P-450, adrenodoxin and adrenodoxin reductase in adrenal cortex and corpus luteum: Implications for membrane organization and gene regulation.
Hanukoglu I., and Hanukoglu Z.
Eur J Biochem 157:27-31, 1986. REPRINT

20. Isolation of a cDNA for adrenodoxin reductase (ferredoxin-NADP+ reductase). Implications for mitochondrial cytochrome P-450 systems.
Hanukoglu I., Gutfinger T., Haniu M., and Shively J.E.
Eur J Biochem 169:449-455, 1987. REPRINT

21. Human adrenodoxin reductase: two mRNAs encoded by a single gene on chromosome 17cen-q25 are expressed in steroidogenic tissues.
Solish S. B., Picado-Leonard J., Morel Y., Kuhn R. W., Mohandas T.K., Hanukoglu I., and Miller W.L.
Proc Natl Acad Sci USA 85:7104-7108, 1988. REPRINT

22. cDNA sequence of adrenodoxin reductase. Identification of NADP-binding sites in oxidoreductases.
Hanukoglu I., and Gutfinger T.
Eur J Biochem 180:479-484, 1989. REPRINT

23. Olfactory-specific cytochrome P-450. cDNA cloning of a novel neuroepithelial enzyme possibly involved in chemoreception.
Nef P., Heldman J., Lazard D., Margalit T., Jaye M., Hanukoglu I., and Lancet D.
J Biol Chem 264:6780-6785, 1989. REPRINT

24. Molecular biology of cytochrome P450 systems in steroidogenic tissues [conference].
Hanukoglu I.
Serono Symposia Review 23:233-252, 1989.

25. Elimination of non-specific binding in western blots from non-reducing gels.
Hanukoglu I.
J Biochem Biophys Methods 21:65-68, 1990. REPRINT

26. Induction and mitochondrial localization of cytochrome P450scc system enzymes in normal and transformed ovarian granulosa cells.
Hanukoglu I., Suh B.S., Himmelhoch S., and Amsterdam A.
J Cell Biol 111:1373-1381, 1990. REPRINT

27. Mechanism of corticotropin and cAMP induction of mitochondrial cytochrome P450 system enzymes in adrenal cortex cells.
Hanukoglu I., Feuchtwanger R., and Hanukoglu A.
J Biol Chem 265:20602-20608, 1990. REPRINT

28. Adrenodoxin reductase of mitochondrial cytochrome P450 systems: structure and regulation of expression [conference].
Hanukoglu I.
Flavins and Flavoproteins 1990 (Curti, B., Ronchi, S., and Zanetti, G., Eds.). pp. :859-864, 1991.

30. Reductases of the bacterial, mitochondrial and microsomal cytochrome P450 systems: structural comparisons [conference].
Hanukoglu I.
Cytochrome P-450: Biochemistry and Biophysics (A.I. Archakov and G.I. Bachmanova, eds.), INCO-TNC, Moscow, pp. :339-344, 1992.

31. Current research on steroid metabolism: transition from biochemistry to molecular-cell biology.
Hanukoglu I.
J Steroid Biochem Molec Biol 43:745-749, 1992. REPRINT

32. Steroidogenic enzymes: structure, function, and role in regulation of steroid hormone biosynthesis.
Hanukoglu I.
J Steroid Biochem Molec Biol 43:779-804, 1992. REPRINT

33. Oncogene-transformed granulosa cells as a model system for the study of steroidogenic processes.
Amsterdam A., Hanukoglu I., Suh B.S., Keren-Tal I., Plehn-Dujowich D., Sprengel R., Rennert H., and Strauss III J.F.
J Steroid Biochem Molec Biol 43:875-884, 1992. REPRINT

34. A molecular view of steroid metabolism.
Hanukoglu I. (editor)
J Steroid Biochem Molec Biol 43:745-1124, 1992. REPRINT

35. Proceedings of the 9th International Symposium on Microsomes and Drug Oxidations.
Kapitulnik J., and Hanukoglu I. (editors).
J Basic Clin Physiol Pharmacol 3:1-309, 1992. REPRINT

36. Electron leakage from the mitochondrial NADPH-adrenodoxin reductase-adrenodoxin-P450scc (cholesterol side chain cleavage) system.
Hanukoglu I., Rapoport R., Weiner L., and Sklan D.
Arch Biochem Biophys 305:489-498, 1993. REPRINT

37. Mitochondrial-genome-encoded RNAs: differential regulation by corticotropin in bovine adrenocortical cells.
Raikhinstein M., and Hanukoglu I.
Proc Natl Acad Sci USA 90:10509-10513, 1993. REPRINT

39. cDNA cloning and sequence analysis of the bovine adrenocorticotropic hormone (ACTH) receptor.
Raikhinstein M., Zohar M., and Hanukoglu I.
Biochim Biophys Acta 1220:329-332, 1994. REPRINT

40. A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems.
Rapoport R., Hanukoglu I., and Sklan D.
Anal Biochem 218:309-313, 1994. REPRINT

41. Cloning of ACTH-regulated genes in the adrenal cortex.
Raikhinstein M., and Hanukoglu I.
J Steroid Biochem Mol Biol 49:257-260, 1994. REPRINT

42. Electron pathways in adrenal mitochondrial cytochrome P450 systems: Relative rates of leakage and hydroxylation [conference].
Rapoport R., Raikhinstein M., Sklan D., and Hanukoglu I.
Cytochrome P-450: Biochemistry Biophysics and Molecular Biology (M. C. Lechner, ed.). John Libbey Eurotext, Paris, pp. :357-363, 1994.

43. Electron leakage from the adrenal cortex mitochondrial P450scc and P450c11 systems: NADPH and steroid dependence.
Rapoport R., Sklan D., and Hanukoglu I.
Arch Biochem Biophys 317:412-416, 1995. REPRINT

44. Routes and regulation of NADPH production in steroidogenic mitochondria.
Hanukoglu I., and Rapoport R.
Endocr Res 21:231-241, 1995. REPRINT

45. Selective increases in adrenal steroidogenic capacity during acute respiratory disease in infants.
Hanukoglu A., Fried D., Nakash I., and Hanukoglu I.
Eur J Endocrinol 133:552-556, 1995. REPRINT

47. Tissue- and species-dependent expression of sheep lung microsomal cytochrome P4502B(LgM2).
Arinc E., Hanukoglu I., Sen A., and Adali O.
Biochem Mol Biol Int 37:1121-1126, 1995.

52. Electron transfer proteins of cytochrome P450 systems.
Hanukoglu I.
Adv Mol Cell Biol 14:29-55, 1996. REPRINT

54. Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle.
Rapoport R., Sklan D., Wolfenson D., Shaham-Albalancy A., and Hanukoglu I.
Biochim Biophys Acta 1380:133-140, 1998. REPRINT

55. In situ localization of ACTH receptor-like mRNA in molluscan and human immunocytes.
Ottaviani E., Franchini A., and Hanukoglu I.
Cell Mol Life Sci 54:139-142, 1998. REPRINT

57. Structures of mitochondrial P450 system proteins [conference].
Hanukoglu I.
Molecular and applied aspects of oxidative drug metabolizing enzymes (E. Arinc, E. Hodgeson and J.B. Schenkman, eds.) Plenum Publishing Corp. pp. :41-54, 1999. DOI

58. Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli.
Vonrhein C., Schmidt U., Ziegler G.A., Schweiger S., Hanukoglu I., and Schulz G.E.
FEBS Lett 443:167-169, 1999. REPRINT

59. The structure of adrenodoxin reductase of mitochondrial P450 systems: electron transfer for steroid biosynthesis.
Ziegler G.A., Vonrhein C., Hanukoglu I., and Schulz G.E.
J Mol Biol 289:981-990, 1999. REPRINT

60. Structure and function of bovine adrenodoxin reductase [conference].
Ziegler G.A., Vonrhein C., Hanukoglu I., and Schulz G.E.
Flavins and Flavoproteins (Ghisla S., Kroneck P., Macheroux P., Sund H., eds.), Rudolf Weber, Berlin, pp. :729-736, 1999.

61. Structures of adrenodoxin reductase and adrenodoxin support shuttle mechanism of electron transfer in mitochondrial P450 systems [conference].
Ziegler G.A., Vonrhein C., Schulz G.E., and Hanukoglu I.
Molecular Steroidogenesis (M. Okamoto, Y. Ishimura, H. Nawata, eds.), Universal Academy Press, Tokyo, Japan, pp. :61-64, 2000. REPRINT

66. ACTH induces TIMP-1 expression and inhibits collagenase in adrenal cortex cells.
Reichenstein M., Reich R., LeHoux J.G., and Hanukoglu I.
Mol. Cell. Endocrinol. 215:109-114, 2004. REPRINT

68. Antioxidant protective mechanisms against reactive oxygen species (ROS) generated by mitochondrial P450 systems in steroidogenic cells (Review).
Hanukoglu I.
Drug Metab Rev 38:171-196, 2006. REPRINT

78. Proteopedia: Rossmann fold: A beta-alpha-beta fold at dinucleotide binding sites.
Hanukoglu I.
Biochem Mol Biol Edu 43:206-209, 2015. REPRINT

84. Conservation of the enzyme-coenzyme interfaces in FAD and NADP binding adrenodoxin reductase - A ubiquitous enzyme.
Hanukoglu I.
J Mol Evol 85:205-218, 2017. REPRINT

Note: References 5 and 7 are the major references for cholesterol side-chain-cleaving cytochrome P450scc in the IUBMB Enzyme Nomenclature